Erice2010:workshop7: Difference between revisions
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This is workshop #7, cryoEM, led by Carsten Sachse from EMBL | |||
== Aim == | |||
The aim of the cryoEM workshop is to cover basic concepts of cryo-EM 3D structure determination with particular focus on single-particle methods. The hands-on session introduces the SPIDER image processing software and applies it to the reference specimen Tobacco Mosaic Virus. | |||
== Outline == | |||
The two-hour workshop comprises | |||
- a theoretical introduction to the topic covering the steps from image acquisition to the 3D structure | |||
- a “watch-me” style presentation of simple image processing operations | |||
- a walkthrough through the processing of a small data set | |||
== Suitability == | |||
This workshop is prepared for complete novices in image processing but some basic knowledge of working with unix-style command line computers would be desirable. As a result of the course you will be able to reconstruct a reference data set of Tobacco Mosaic Virus. Because of the diversity of distinct samples and image processing approaches, we will focus on fundamental aspects of the procedure. | |||
-- Carsten Sachse, May 7 2010 | |||
[[Portal:Erice2010 | return to main workshop page]] | [[Portal:Erice2010 | return to main workshop page]] | ||
[[Category:Erice2010]] | [[Category:Erice2010]] | ||
Latest revision as of 20:17, 8 October 2012
This is workshop #7, cryoEM, led by Carsten Sachse from EMBL
Aim
The aim of the cryoEM workshop is to cover basic concepts of cryo-EM 3D structure determination with particular focus on single-particle methods. The hands-on session introduces the SPIDER image processing software and applies it to the reference specimen Tobacco Mosaic Virus.
Outline
The two-hour workshop comprises
- a theoretical introduction to the topic covering the steps from image acquisition to the 3D structure
- a “watch-me” style presentation of simple image processing operations
- a walkthrough through the processing of a small data set
Suitability
This workshop is prepared for complete novices in image processing but some basic knowledge of working with unix-style command line computers would be desirable. As a result of the course you will be able to reconstruct a reference data set of Tobacco Mosaic Virus. Because of the diversity of distinct samples and image processing approaches, we will focus on fundamental aspects of the procedure.
-- Carsten Sachse, May 7 2010