Contract Research Organizations

Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.

PDSP

The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.

To submit compounds for screening:

1. Login to the PDSP.
2. Create a new request

• • Provide the grant number, name name, known activity and rational
  • Add the compounds and requested assays

Here the address to send compounds (good as of 7/2014)

   PDSP
   Attn: Xi-Ping Huang
   120 Mason Farm Rd
   2113 Genetic Medicine Bldg
   Chapel Hill, NC 27599
   TEL: (919)-966-8777

When sending compounds provide:

• Shoichet Lab
• project name
• compound id, amount submitted, molecular weight
• label the vials with the ID/name and the PDSP# assigned

Eurofins Panlabs

Located in Taiwan, they have 1,350 assays covering the following target classes

• Cytokine/Chemokine (41)
• GPCR (270)
• Ion Channel (41)
• Kinase (154)
• Nuclear Receptor (16)
• Other (15)
• Phosphatase (31)
• Phosphodiesterase (19)
• Protease (51)
• Transporter (19)

They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.

The shoichet lab contact there has been (10/14):

   Judy Winslow, phone #: 425-785-3736, email: JudyWinslow@eurofins.com.

Reaction Biology

Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:

• HMT/DNMT Assays
• HDAC/HAT Assays
• Bromodomain Assays
• DUB Assays
• Protease Assays
• PDE Assays
• CYP/hERG/Bcl/HSP90 Assays
• Cell Assays
• Phosphatase Assays

Their tag line is With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

• ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)
• They have a minimum order of 300USD order

CarnaBio

Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in (Laggner NatChemBio 2012).

Multispan

In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.

ChanTest - Now part of Charles River

In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable. They've now been acquired by Charles River.

ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.

Aurora Biomed

Aurora’s product range includes automated liquid-handling equipment, atomic absorption spectrometers, atomic fluorescence spectrometers, ion channel screening technology and microwave digestion systems, increasing the efficiency of sample management in a wide range of research applications. We are headquartered in Vancouver, BC, Canada.

Matt worked with Andy Hegel (andrew.hegle@aurorabiomed.com) to test compounds with patch-clamp assays for the Systems SEA project in 2014. They gave us a discount of 200$ per datapoint.

PerkinElmer

Large collection of in vitro services including:

• Cytochrome P450
• Cytokine/Chemokine Secretion
• GPCRs
• Ion Channels (Ligand- & Voltage-Gated)
• Kinases
• Nuclear Receptors
• Other - Enzymes
• Other - Receptors
• Phosphodiesterases (PDEs)
• Transporters

and some in vivo services

Complete Assay Services List

Optivia

Specialized in transporters, based in the Bay Area.

Solvo

Specialized in transporters, based in the Bay Area.

GenScript

They have few assays for different classes of targets

BPS Biosciences

Mostly enzymes

InvivoGen

Located in San Diego, focusing on immune response

Assays for Pattern Recognition Receptor activity

• Toll-Like Receptors
• NOD-Like Receptors
• RIG-I-Like Receptors
• C-type Lectin Receptors
• Cytosolic dsDNA Sensors
• Inflammasome
• Autophagy & Innate Immunity
• PRR & PAMPs Detection

INDIGO Biosciences

Located in Pennsylvania, they can do nuclear hormone assay.

Sora Labs

It looks like they have some enzyme assays that they can run.

FlouFarma

Located in France, high content screening of live cells

Cytoskeleton

Compound screening against tubulin, microtubule, myosin, sarcomere, kinesin and small G-proteins.

Other CROs

• Ricerca
• Absorption Biosystems
• amsbio not really a CRO but apparently they can do assays