Contract Research Organizations

Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.

PDSP

The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.

To submit compounds for screening:

1. Login to the PDSP.
2. Create a new request

• • Provide the grant number, name name, known activity and rational
  • Add the compounds and requested assays

Here the address to send compounds (good as of 7/2014)

   PDSP
   attn Flor Sassa
   120 Mason Farm Rd
   2113 Genetic Medicine Bldg
   Chapel Hill, NC 27599
   TEL: (919)-966-8777

When sending compounds provide:

   Shoichet Lab
   project name
   compound id, amount submitted, molecular weight, and pdsp id assigned

Eurofins Panlabs

Located in Taiwan, they have 1,350 assays covering the following target classes

• Cytokine/Chemokine (41)
• GPCR (270)
• Ion Channel (41)
• Kinase (154)
• Nuclear Receptor (16)
• Other (15)
• Phosphatase (31)
• Phosphodiesterase (19)
• Protease (51)
• Transporter (19)

They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.

Reaction Biology

Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:

• HMT/DNMT Assays
• HDAC/HAT Assays
• Bromodomain Assays
• DUB Assays
• Protease Assays
• PDE Assays
• CYP/hERG/Bcl/HSP90 Assays
• Cell Assays
• Phosphatase Assays

Their tag line is With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

• ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)
• They have a minimum order of 300USD order

CarnaBio

Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in (Laggner NatChemBio 2012).

Multispan

In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.

ChanTest

In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.

ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.

PerkinElmer

Large collection of in vitro services including:

• Cytochrome P450
• Cytokine/Chemokine Secretion
• GPCRs
• Ion Channels (Ligand- & Voltage-Gated)
• Kinases
• Nuclear Receptors
• Other - Enzymes
• Other - Receptors
• Phosphodiesterases (PDEs)
• Transporters

and some in vivo services

Complete Assay Services List

Other CROs

• Ricerca