Wendywilson: There are several viral gene delivery systems, including adenovirus, retrovirus and lentivirus. Which one should I use for my experiments? 1) Retrovirus: requires active cell division. In addition, there is a significant risk of integration into the h...

2018-03-02T03:45:29Z

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There are several viral gene delivery systems, including adenovirus, retrovirus and lentivirus. Which one should I use for my experiments?

1) Retrovirus: requires active cell division. In addition, there is a significant risk of integration into the host genome, leading to mutation of genes or activation of onco-genes in the host system, which is a concern for scientists.
2) Lentivirus: can infect both dividing and non-dividing cells. Like retrovirus, there is a significant risk of integration into the host genome, leading to mutation of genes or activation of oncogenes in the host system.
3) Adenovirus: 100% gene delivery efficiency in most cell types including dividing and non-dividing or primary cells. There is no integration with the host system.
4) AAV: has the capacity to produce high titer virus with broad spectrum of tropism in dividing and non-dividing cells and potential for long-term gene transfer with minimum immnunogenicity.
https://www.creative-biogene.com/Support/Virus.html

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Wendywilson: There are several viral gene delivery systems, including adenovirus, retrovirus and lentivirus. Which one should I use for my experiments? 1) Retrovirus: requires active cell division. In addition, there is a significant risk of integration into the h...