User contributions for Wendywilson
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2 March 2018
- 03:5003:50, 2 March 2018 diff hist +940 N File:Cas9 stable cell line.png CRISPR/Cas9 system used for genome engineering in molecular biology is composed of two components: sgRNA which is a combine of original trRNA and crRNA, and Cas9 endonuclease. By delivering Cas9 and sgRNA into a cell, Cas9 is targeted to a given locus ... current
- 03:4803:48, 2 March 2018 diff hist +694 N File:RcAAV Testing.png While AAV vectors are engineered to be replication defective, generation of replication-competent AAV (rcAAV) can still occur during vector manufacturing by means of recombination events within the producer cells. rcAAV may develop at any step during t... current
- 03:4703:47, 2 March 2018 diff hist +362 N File:Limiting dilution.png Limiting dilution analysis attempts to determine the frequency of cells having a particular function that are present in a mixed population of cells. So limiting dilution analysis is an easy-operated method that used to measure the abundance of cells a... current
- 03:4603:46, 2 March 2018 diff hist +629 N File:Lentivirus transduction.png How can lentiviral vectors be used to make stable cell lines? Many lentiviral vectors insert some selectable markers, such as the puromycin resistance gene, conferring resistance to antibiotics. If these antibiotics are added to the growth medium, they... current
- 03:4503:45, 2 March 2018 diff hist +1,007 N File:Custom Virus.png There are several viral gene delivery systems, including adenovirus, retrovirus and lentivirus. Which one should I use for my experiments? 1) Retrovirus: requires active cell division. In addition, there is a significant risk of integration into the h... current
- 03:4303:43, 2 March 2018 diff hist +406 N File:Synthesize gene.png What is the difference between a long oligo and a synthetic gene? Answer: Due to limitations in oligo synthesis chemistry and other practical considerations, oligos are produced as mixture of single-stranded DNA strands and are limited in length to ~20... current
- 03:4203:42, 2 March 2018 diff hist +219 N File:Gene service.png How do you deliver synthetic genes? Answer: Creative Biogene will deliver 5.0ug plasmid DNA containing gene of interest in the vector and its bacterial stock. https://www.creative-biogene.com/Support/Gene-Synthesis.html current
- 03:4103:41, 2 March 2018 diff hist +567 N File:3 UTR Cloning.png Creative Biogene has developed an advanced vector-based miRNA expression plasmids, which can help to ease the difficulty in your research. These purified and ready to transfect miRNA expression plasmids can be used for a variety of applications to down... current
2 January 2018
- 04:2604:26, 2 January 2018 diff hist +552 N File:Ion Channel Function.png Manual patch clamp is considered the ‘gold standard’ for assessing [https://www.creative-biogene.com/Services/Drug-Discovery-Services/Ion-Channel-Screening-and-profiling-with-Functional-Assays.html ion channel function]. It measures biophysical and... current
- 04:2304:23, 2 January 2018 diff hist +247 N File:Synthesis gene.png Artificial [https://www.creative-biogene.com/Services/Gene-Service/Gene-Synthesis-and-cloning-service.html gene synthesis], sometimes known as DNA printing is a method in synthetic biology that is used to create artificial genes in the laboratory. current
- 04:2204:22, 2 January 2018 diff hist +281 N File:Cell line knockout.png Only 1 sgRNA is used to achieve [https://www.creative-biogene.com/Services/Stable-cell-line-generation/Custom-Genome-Editing-Cell-Lines.html cell line knockout]. In the case that the first sgRNA does not allow for success, we would try another from the... current
- 04:2004:20, 2 January 2018 diff hist +295 N File:GMP virus.png During a period of development, Creative Biogene has already possessed sophisticated equipments, advanced technologies and highly experienced staffs which are available to provide you with [https://www.creative-biogene.com/Services/Custom-Viral-Service... current
