http://wiki.docking.org/api.php?action=feedcontributions&feedformat=atom&user=SbarelierDISI - User contributions [en]2026-05-24T08:54:51ZUser contributionsMediaWiki 1.39.1http://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=8241Contract Research Organizations2015-01-27T23:20:24Z<p>Sbarelier: </p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here is the address to send compounds vis FedEx (good as of 11/2014)<br />
<br />
PDSP<br />
Attn: Xi-Ping Huang<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27514<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
* Shoichet Lab<br />
* project name<br />
* compound id, amount submitted, molecular weight (they usually would like at least 10 mg)<br />
* label the vials with the ID/name and the PDSP# assigned (they will send you labels)<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
The shoichet lab contact there has been (10/14):<br />
Judy Winslow, phone #: 425-785-3736, email: JudyWinslow@eurofins.com.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest - Now part of Charles River]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
They've now been acquired by Charles River.<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.aurorabiomed.com/ion-channel-screening-assays/ Aurora Biomed]==<br />
Aurora’s product range includes automated liquid-handling equipment, atomic absorption spectrometers, atomic fluorescence spectrometers, ion channel screening technology and microwave digestion systems, increasing the efficiency of sample management in a wide range of research applications. We are headquartered in Vancouver, BC, Canada.<br />
<br />
Matt worked with Andy Hegel (andrew.hegle@aurorabiomed.com) to test compounds with patch-clamp assays for the Systems SEA project in 2014. They gave us a discount of 200$ per datapoint.<br />
<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
AS OF JAN 1 2015 THEY ARE DISCONTINUING THEIR ASSAY SERVICES DIVISION<br />
<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.solvobiotech.com/ Solvo]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.genscript.com/bio-assay_center.html GenScript]==<br />
They have few assays for different classes of targets<br />
<br />
==[http://bpsbioscience.com/ BPS Biosciences]==<br />
Mostly enzymes<br />
<br />
==[http://www.invivogen.com/innate-immunity InvivoGen]==<br />
Located in San Diego, focusing on immune response<br />
<br />
Assays for Pattern Recognition Receptor activity<br />
* Toll-Like Receptors<br />
* NOD-Like Receptors<br />
* RIG-I-Like Receptors<br />
* C-type Lectin Receptors<br />
* Cytosolic dsDNA Sensors<br />
* Inflammasome<br />
* Autophagy & Innate Immunity<br />
* PRR & PAMPs Detection<br />
<br />
==[http://indigobiosciences.com/screening-services/nuclear-receptor-assays/ INDIGO Biosciences]==<br />
Located in Pennsylvania, they can do nuclear hormone assay.<br />
<br />
==[http://www.soralabs.com/services/ Sora Labs]==<br />
It looks like they have some enzyme assays that they can run.<br />
<br />
==[http://www.fluofarma.com/ FlouFarma]==<br />
Located in France, high content screening of live cells<br />
<br />
==[http://www.cytoskeleton.com/custom-services/compound-screening Cytoskeleton]==<br />
Compound screening against tubulin, microtubule, myosin, sarcomere, kinesin and small G-proteins.<br />
<br />
==[https://www.caymanchem.com/app/template/services%2CHome.vm Cayman Chemical]==<br />
* Assay Services<br />
Biochemists with experience in the areas of eicosanoids, steroids, oxidative stress, and inhibitor screening<br />
Use Cayman’s extensive line of well-characterized biochemical kits<br />
Enzyme immunoassay, colorimetric, and fluorometric assays available<br />
* Screening Services<br />
Comprehensive screening laboratory focused on epigenetics<br />
Backed by our core strengths in protein production, assay development, chemical synthesis, and medicinal chemistry<br />
Capacity to test compounds at a rate of up to 100,000 compounds per week<br />
Ability to profile compounds against a broad epigenetic enzyme panel<br />
Includes assays for methyltransferases, demethylases, acetyltransferases, deacetylases (HDACs and SIRTS), and histone code readers (ex. bromodomain proteins)<br />
<br />
==[http://www.discoverx.com/ DiscoveRx]==<br />
GPCR, Kinases, Nuclear Receptors, Epigenetic Targets and Signaling Pathways<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]<br />
* [http://www.absorption.com Absorption Biosystems]<br />
* [http://www.amsbio.com/ amsbio] not really a CRO but apparently they can do assays<br />
* [http://stores.arraybridge.com/enzyme-assays/ ArrayBridge] The have ~850 enzymes listed that they say they have assays for, but little detail on what services they actually provide.</div>Sbarelierhttp://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=8189Contract Research Organizations2014-11-12T01:00:14Z<p>Sbarelier: </p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here the address to send compounds (good as of 7/2014)<br />
<br />
PDSP<br />
Attn: Xi-Ping Huang<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27599<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
* Shoichet Lab<br />
* project name<br />
* compound id, amount submitted, molecular weight<br />
* label the vials with the ID/name and the PDSP# assigned<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
The shoichet lab contact there has been (10/14):<br />
Judy Winslow, phone #: 425-785-3736, email: JudyWinslow@eurofins.com.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest - Now part of Charles River]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
They've now been acquired by Charles River.<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.aurorabiomed.com/ion-channel-screening-assays/ Aurora Biomed]==<br />
Aurora’s product range includes automated liquid-handling equipment, atomic absorption spectrometers, atomic fluorescence spectrometers, ion channel screening technology and microwave digestion systems, increasing the efficiency of sample management in a wide range of research applications. We are headquartered in Vancouver, BC, Canada.<br />
<br />
Matt worked with Andy Hegel (andrew.hegle@aurorabiomed.com) to test compounds with patch-clamp assays for the Systems SEA project in 2014. They gave us a discount of 200$ per datapoint.<br />
<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.solvobiotech.com/ Solvo]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.genscript.com/bio-assay_center.html GenScript]==<br />
They have few assays for different classes of targets<br />
<br />
==[http://bpsbioscience.com/ BPS Biosciences]==<br />
Mostly enzymes<br />
<br />
==[http://www.invivogen.com/innate-immunity InvivoGen]==<br />
Located in San Diego, focusing on immune response<br />
<br />
Assays for Pattern Recognition Receptor activity<br />
* Toll-Like Receptors<br />
* NOD-Like Receptors<br />
* RIG-I-Like Receptors<br />
* C-type Lectin Receptors<br />
* Cytosolic dsDNA Sensors<br />
* Inflammasome<br />
* Autophagy & Innate Immunity<br />
* PRR & PAMPs Detection<br />
<br />
==[http://indigobiosciences.com/screening-services/nuclear-receptor-assays/ INDIGO Biosciences]==<br />
Located in Pennsylvania, they can do nuclear hormone assay.<br />
<br />
==[http://www.soralabs.com/services/ Sora Labs]==<br />
It looks like they have some enzyme assays that they can run.<br />
<br />
==[http://www.fluofarma.com/ FlouFarma]==<br />
Located in France, high content screening of live cells<br />
<br />
==[http://www.cytoskeleton.com/custom-services/compound-screening Cytoskeleton]==<br />
Compound screening against tubulin, microtubule, myosin, sarcomere, kinesin and small G-proteins.<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]<br />
* [http://www.absorption.com Absorption Biosystems]<br />
* [http://www.amsbio.com/ amsbio] not really a CRO but apparently they can do assays</div>Sbarelierhttp://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=8187Contract Research Organizations2014-11-10T23:00:08Z<p>Sbarelier: /* INDIGO Biosciences */</p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here the address to send compounds (good as of 7/2014)<br />
<br />
PDSP<br />
Attn: Xi-Ping Huang<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27599<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
* Shoichet Lab<br />
* project name<br />
* compound id, amount submitted, molecular weight<br />
* label the vials with the ID/name and the PDSP# assigned<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
The shoichet lab contact there has been (10/14):<br />
Judy Winslow, phone #: 425-785-3736, email: JudyWinslow@eurofins.com.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest - Now part of Charles River]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
They've now been acquired by Charles River.<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.aurorabiomed.com/ion-channel-screening-assays/ Aurora Biomed]==<br />
Aurora’s product range includes automated liquid-handling equipment, atomic absorption spectrometers, atomic fluorescence spectrometers, ion channel screening technology and microwave digestion systems, increasing the efficiency of sample management in a wide range of research applications. We are headquartered in Vancouver, BC, Canada.<br />
<br />
Matt worked with Andy Hegel (andrew.hegle@aurorabiomed.com) to test compounds with patch-clamp assays for the Systems SEA project in 2014. They gave us a discount of 200$ per datapoint.<br />
<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.solvobiotech.com/ Solvo]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.genscript.com/bio-assay_center.html GenScript]==<br />
They have few assays for different classes of targets<br />
<br />
==[http://bpsbioscience.com/ BPS Biosciences]==<br />
Mostly enzymes<br />
<br />
==[http://www.invivogen.com/innate-immunity InvivoGen]==<br />
Located in San Diego, focusing on immune response<br />
<br />
Assays for Pattern Recognition Receptor activity<br />
* Toll-Like Receptors<br />
* NOD-Like Receptors<br />
* RIG-I-Like Receptors<br />
* C-type Lectin Receptors<br />
* Cytosolic dsDNA Sensors<br />
* Inflammasome<br />
* Autophagy & Innate Immunity<br />
* PRR & PAMPs Detection<br />
<br />
==[http://indigobiosciences.com/screening-services/nuclear-receptor-assays/ INDIGO Biosciences]==<br />
Located in Pennsylvania, they can do nuclear hormone assay.<br />
<br />
==[http://www.soralabs.com/services/ Sora Labs]==<br />
It looks like they have some enzyme assays that they can run.<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]<br />
* [http://www.absorption.com Absorption Biosystems]<br />
* [http://www.amsbio.com/ amsbio] not really a CRO but apparently they can do assays</div>Sbarelierhttp://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=8186Contract Research Organizations2014-11-10T22:19:06Z<p>Sbarelier: /* ChanTest */</p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here the address to send compounds (good as of 7/2014)<br />
<br />
PDSP<br />
Attn: Xi-Ping Huang<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27599<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
* Shoichet Lab<br />
* project name<br />
* compound id, amount submitted, molecular weight<br />
* label the vials with the ID/name and the PDSP# assigned<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
The shoichet lab contact there has been (10/14):<br />
Judy Winslow, phone #: 425-785-3736, email: JudyWinslow@eurofins.com.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest - Now part of Charles River]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
They've now been acquired by Charles River.<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.aurorabiomed.com/ion-channel-screening-assays/ Aurora Biomed]==<br />
Aurora’s product range includes automated liquid-handling equipment, atomic absorption spectrometers, atomic fluorescence spectrometers, ion channel screening technology and microwave digestion systems, increasing the efficiency of sample management in a wide range of research applications. We are headquartered in Vancouver, BC, Canada.<br />
<br />
Matt worked with Andy Hegel (andrew.hegle@aurorabiomed.com) to test compounds with patch-clamp assays for the Systems SEA project in 2014. They gave us a discount of 200$ per datapoint.<br />
<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.solvobiotech.com/ Solvo]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.genscript.com/bio-assay_center.html GenScript]==<br />
They have few assays for different classes of targets<br />
<br />
==[http://bpsbioscience.com/ BPS Biosciences]==<br />
Mostly enzymes<br />
<br />
==[http://www.invivogen.com/innate-immunity InvivoGen]==<br />
Located in San Diego, focusing on immune response<br />
<br />
Assays for Pattern Recognition Receptor activity<br />
* Toll-Like Receptors<br />
* NOD-Like Receptors<br />
* RIG-I-Like Receptors<br />
* C-type Lectin Receptors<br />
* Cytosolic dsDNA Sensors<br />
* Inflammasome<br />
* Autophagy & Innate Immunity<br />
* PRR & PAMPs Detection<br />
<br />
==[http://indigobiosciences.com/screening-services/nuclear-receptor-assays/ INDIGO Biosciences]==<br />
Located in Pennsylvania, they have can do nuclear hormone assay.<br />
<br />
==[http://www.soralabs.com/services/ Sora Labs]==<br />
It looks like they have some enzyme assays that they can run.<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]<br />
* [http://www.absorption.com Absorption Biosystems]<br />
* [http://www.amsbio.com/ amsbio] not really a CRO but apparently they can do assays</div>Sbarelierhttp://wiki.docking.org/index.php?title=Assaying_Compound_Activity&diff=8145Assaying Compound Activity2014-10-24T21:37:59Z<p>Sbarelier: /* Testing Compounds with CRO */</p>
<hr />
<div>Depending on the project, assaying compound activity typically involves acquiring the compound and then getting it tested either in house or sending it out somewhere to be tested. This page details some of the common tasks and considerations in this process. Note, this information is somewhat specific to the Shoichet lab at UCSF.<br />
<br />
<br />
=Buying Compounds=<br />
<br />
==Identifying Compounds to Buy==<br />
Depending on the project, compounds may have different requirements<br />
*Requirements for in vitro testing<br />
** Purity: If possible get the compounds at 95% or greater purity even if it costs 100$ or 200$ more. If it's tested to be active but has low purity, it will likely need to be re-purified and then tested.<br />
** Aggregation: Check the compound with the aggregator advisor (advisor.bkslab.org) and if it's close to or known to be an aggregator (indications include uM nonspecific activity, logP great than 3 or actually shown to be an aggregator through appropriate controls) then either skip the compound or plan on doing aggregation controls, e.g. testing for detergent sensitive nonspecific activity, determine particle formation using DLS, or perform the assay with/without detergent (and just detergent controls).<br />
** Amount: try to get 10mg if possible, but if it's very expensive you can get less. If you're sending it to a CRO sending it in solution usually requires less compound. If you're planning on further controls (e.g. for purity, aggregation), make sure to get enough.<br />
** Note the solubility (e.g. water, DMSO, etc.)<br />
<br />
*Requirements for in vivo testing<br />
** Check pharmacokinetics<br />
<br />
==Finding Vendors==<br />
The easiest way to find vendors is through the ZINC portal. Once you login, go to Manage Carts in the upper right and then create new cart, where you put in a list of smiles or zinc ids. Once you've verified these are the right compounds, click purchasability report where it lists which compounds are available from which vendors. In selecting vendors keep the following in mind<br />
* Here are some reliable vendors<br />
** AK Sientific (in SF)<br />
** Toronto Research Chemicals<br />
** Sigma Aldrich (sometimes higher prices but reliable)<br />
** Enamine (has a large catalog, in europe)<br />
** ChemBridge (in SF)<br />
** Santa Cruz Biotech (in CA)<br />
* Some vendors are just resellers, which makes them have larger catalogs, but can increase the price and take longer. Though often this is offset by the ease of use and not having to pay multiple shipping and handling costs.<br />
** MolPort (ieva.kluga@molport.com has been the contact as of July 2014)<br />
** EMolecules<br />
** Ambinter<br />
* If possible try to group multiple compounds per vendor to reduce shipping and handling costs<br />
* Sometimes compounds are available from special catalogs<br />
** NCI Platted: 1) Here is a description of the NCI Platted process from there end [[NCI_Platted]]. Look up the NCS identifier in PubChem. 2) submit a request form through their webpage. Contact the industry contacts officer at ucsf (Esteban.Mendoza@ucsf.edu | t: 415-502-1599) to have him sign the "Simple Letter of Agreement". He'll will give you a materials transfer form that you need to fill out.<br />
* Remember that our lab will continue to buy from these vendors so be nice, and inquire if we can get a lab discount<br />
* For each compound record<br />
** ZincID, supplier, supplier product code, molecular weight, purity<br />
<br />
==Ordering Compounds==<br />
* Get quotes either through the webpage or through vendor contacts<br />
* Get Brian to approve the orders costing more than 500USD.<br />
* Forward quotes to Yvonne or whoever is doing the ordering <br />
*** Be cognizant of the hours the orderer works, sometimes they share time with other labs<br />
*** the quote should including the zincid, vendor, vendor id, amount, and price<br />
*** lookup the project [[grant number]] (or ask Brian),<br />
*** a shipping address<br />
*** whose project this is for <br />
*** who should receive the compounds<br />
*** indicate if the order should be rushed (unless necessary, rushed orders should be avoided as they are generally much more expensive)<br />
<br />
==Testing Compounds with CRO==<br />
<br />
Here is a list of CROs [http://wiki.bkslab.org/index.php?title=Contract_Research_Organizations].<br />
*Quote administration<br />
** If the vendor is not in the BearBuy system, they will need to fill out a form that Yvonne can provide<br />
** Get a quote from the company and<br />
** With the quote, get a PO number<br />
** Give the PO number to the vendor when placing the order<br />
** The vendor will then give an Invoice that can go directly to the UCSF Accounts Payable department (COapmail@ucsf.edu) and they will pay the vendor.<br />
*Sending compounds<br />
** Provide the courier (e.g. FedEx) with the address and full name and telephone number of the person who will receive it.<br />
** Indicate with the vendor if the order should be rushed (unless necessary, rushed orders should be avoided as they are generally much more expensive)<br />
** If the supply of the compound is limited, often less is required if it is sent in solution, but it is easier and more stable to send powder, so check with the assay vendor<br />
* Compound testing<br />
** For the given receptor/ligand pair check the known activities to help guide where to start testing.<br />
** If possible, start with a few high concentration (e.g. 2 replicates @ 20 uM) "primary" points to see if there is any activity<br />
** If there is some activity follow up with a 5-10 point dose response curve reducing by half log or 1/3 at each step (where the highest concentrations will depend on the activity of the primary assay).<br />
<br />
[[Category:Tutorials]]</div>Sbarelierhttp://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=8125Contract Research Organizations2014-10-17T22:38:20Z<p>Sbarelier: </p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here the address to send compounds (good as of 7/2014)<br />
<br />
PDSP<br />
attn Flor Sassa<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27599<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
* Shoichet Lab<br />
* project name<br />
* compound id, amount submitted, molecular weight<br />
* label the vials with the ID/name and the PDSP# assigned<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.aurorabiomed.com/ion-channel-screening-assays/ Aurora Biomed]==<br />
Aurora’s product range includes automated liquid-handling equipment, atomic absorption spectrometers, atomic fluorescence spectrometers, ion channel screening technology and microwave digestion systems, increasing the efficiency of sample management in a wide range of research applications. We are headquartered in Vancouver, BC, Canada.<br />
<br />
Matt worked with Andy Hegel (andrew.hegle@aurorabiomed.com) to test compounds with patch-clamp assays for the Systems SEA project in 2014. They gave us a discount of 200$ per datapoint.<br />
<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.solvobiotech.com/ Solvo]==<br />
Specialized in transporters, based in the Bay Area.<br />
<br />
==[http://www.genscript.com/bio-assay_center.html GenScript]==<br />
They have few assays for different classes of targets<br />
<br />
==[http://bpsbioscience.com/ BPS Biosciences]==<br />
Mostly enzymes<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]<br />
* [http://www.absorption.com Absorption Biosystems]<br />
* [http://www.amsbio.com/ amsbio] not really a CRO but apparently they can do assays</div>Sbarelierhttp://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=8005Contract Research Organizations2014-08-25T17:31:05Z<p>Sbarelier: /* Other CROs */</p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here the address to send compounds (good as of 7/2014)<br />
<br />
PDSP<br />
attn Flor Sassa<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27599<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
Shoichet Lab<br />
project name<br />
compound id, amount submitted, molecular weight, and pdsp id assigned<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
Specialized in Transporters, based in the Bay Area.<br />
<br />
==[http://www.solvobiotech.com/ Solvo]==<br />
Specialized in Transporters, based in the Bay Area.<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]<br />
* [http://www.absorption.com Absorption Biosystems]</div>Sbarelierhttp://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=8004Contract Research Organizations2014-08-25T17:25:17Z<p>Sbarelier: </p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here the address to send compounds (good as of 7/2014)<br />
<br />
PDSP<br />
attn Flor Sassa<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27599<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
Shoichet Lab<br />
project name<br />
compound id, amount submitted, molecular weight, and pdsp id assigned<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
Specialized in Transporters, based in the Bay Area.<br />
<br />
==[http://www.solvobiotech.com/ Solvo]==<br />
Specialized in Transporters, based in the Bay Area.<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]</div>Sbarelierhttp://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=7982Contract Research Organizations2014-08-19T21:58:35Z<p>Sbarelier: /* Optivia */</p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here the address to send compounds (good as of 7/2014)<br />
<br />
PDSP<br />
attn Flor Sassa<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27599<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
Shoichet Lab<br />
project name<br />
compound id, amount submitted, molecular weight, and pdsp id assigned<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
<br />
Specialized in Transporters<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]</div>Sbarelierhttp://wiki.docking.org/index.php?title=Contract_Research_Organizations&diff=7981Contract Research Organizations2014-08-19T21:58:17Z<p>Sbarelier: </p>
<hr />
<div>Contract research organizations are companies that will perform specific in vitro and in vivo assays to testing the activity of compounds or therapies. Listed here are companies that the Shoichet lab has either worked with or considered working with in the past.<br />
<br />
==[http://pdsp.med.unc.edu PDSP]==<br />
The NIMH Psychoactive Drug Screening Program run by the Roth lab at UNC Chapel Hill, has been a close collaborator in performing in vitro binding and functional assays for almost all GPCR targets and and several transporters. If the PDSP has the assay, they are typically our first choice.<br />
<br />
To submit compounds for screening:<br />
# Login to the [https://pdspdb.unc.edu/html/pdspV2/web/index.php PDSP].<br />
# Create a new request<br />
** Provide the grant number, name name, known activity and rational<br />
** Add the compounds and requested assays<br />
<br />
Here the address to send compounds (good as of 7/2014)<br />
<br />
PDSP<br />
attn Flor Sassa<br />
120 Mason Farm Rd<br />
2113 Genetic Medicine Bldg<br />
Chapel Hill, NC 27599<br />
TEL: (919)-966-8777<br />
<br />
When sending compounds provide:<br />
Shoichet Lab<br />
project name<br />
compound id, amount submitted, molecular weight, and pdsp id assigned<br />
<br />
==[https://www.eurofinspanlabs.com/Panlabs Eurofins Panlabs]==<br />
Located in Taiwan, they have 1,350 assays covering the following target classes<br />
*Cytokine/Chemokine (41)<br />
*GPCR (270)<br />
*Ion Channel (41)<br />
*Kinase (154)<br />
*Nuclear Receptor (16)<br />
*Other (15)<br />
*Phosphatase (31)<br />
*Phosphodiesterase (19)<br />
*Protease (51)<br />
*Transporter (19)<br />
They were used by both Henry Lin and Matthew O'Meara (2013-2014) and they gave reasonable results at reasonable prices.<br />
<br />
==[http://www.reactionbiology.com Reaction Biology]==<br />
Located in Pennsylvania, Reaction Biology specializes in Kinase assays and have a few epigenetic and other assays:<br />
*HMT/DNMT Assays<br />
*HDAC/HAT Assays<br />
*Bromodomain Assays<br />
*DUB Assays<br />
*Protease Assays<br />
*PDE Assays<br />
*CYP/hERG/Bcl/HSP90 Assays<br />
*Cell Assays<br />
*Phosphatase Assays<br />
<br />
Their tag line is ''With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.''<br />
<br />
* ~104.00USD for 10 dose IC50 singlet curve (with 20% academic discount)<br />
*They have a minimum order of 300USD order<br />
<br />
==[http://www.carnabio.com/english CarnaBio]==<br />
Located in Japan, CarnBio has an extensive Kinase pannel of 311 targets and a 2-week turn around. Christian Laggner used them for the kinase assays in [http://www.nature.com/nchembio/journal/v8/n2/abs/nchembio.732.html (Laggner NatChemBio 2012)].<br />
<br />
==[http://www.multispaninc.com Multispan]==<br />
In Hayward CA, Multispan 230 validated GPCR assays. 320 human GPCR and 180 murine GPCR cDNAs are cloned for custom assays. Apparently Mike Keiser has used them, though if PDSP has the target/assay that is the way to go.<br />
<br />
==[http://www.chantest.com ChanTest]==<br />
In Cleveland OH, they have a range of Ion Channel assays. Brian says we've used them before and they were reasonable.<br />
<br />
<br />
''ChanTest has developed the world’s most complete library of validated human ion channel-expressing cell lines to serve all the ion channel needs of its pharmaceutical and biotech customers.''<br />
<br />
==[http://www.perkinelmer.com/ PerkinElmer]==<br />
Large collection of in vitro services including:<br />
* Cytochrome P450<br />
* Cytokine/Chemokine Secretion<br />
* GPCRs<br />
* Ion Channels (Ligand- & Voltage-Gated)<br />
* Kinases<br />
* Nuclear Receptors<br />
* Other - Enzymes<br />
* Other - Receptors<br />
* Phosphodiesterases (PDEs)<br />
* Transporters<br />
and some in vivo services<br />
<br />
[http://www.perkinelmer.com/Catalog/Category/ID/Complete-Services-Assay-List Complete Assay Services List]<br />
<br />
==[http://optiviabio.com/ Optivia]==<br />
<br />
==Other CROs==<br />
* [http://www.ricerca.com Ricerca]</div>Sbarelier