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  • 14:30, 1 October 2018 Chasemwebb (Talk | contribs) uploaded "File:Filter ligands image1.png" (Navigating ZINC15 by gene name. Use the UNIPROT geneID to find a specific target.)
  • 20:50, 1 March 2018 Wendywilson (Talk | contribs) uploaded "File:Cas9 stable cell line.png" (CRISPR/Cas9 system used for genome engineering in molecular biology is composed of two components: sgRNA which is a combine of original trRNA and crRNA, and Cas9 endonuclease. By delivering Cas9 and sgRNA into a cell, Cas9 is targeted to a given locus ...)
  • 20:48, 1 March 2018 Wendywilson (Talk | contribs) uploaded "File:RcAAV Testing.png" (While AAV vectors are engineered to be replication defective, generation of replication-competent AAV (rcAAV) can still occur during vector manufacturing by means of recombination events within the producer cells. rcAAV may develop at any step during t...)
  • 20:47, 1 March 2018 Wendywilson (Talk | contribs) uploaded "File:Limiting dilution.png" (Limiting dilution analysis attempts to determine the frequency of cells having a particular function that are present in a mixed population of cells. So limiting dilution analysis is an easy-operated method that used to measure the abundance of cells a...)
  • 20:46, 1 March 2018 Wendywilson (Talk | contribs) uploaded "File:Lentivirus transduction.png" (How can lentiviral vectors be used to make stable cell lines? Many lentiviral vectors insert some selectable markers, such as the puromycin resistance gene, conferring resistance to antibiotics. If these antibiotics are added to the growth medium, they...)
  • 20:45, 1 March 2018 Wendywilson (Talk | contribs) uploaded "File:Custom Virus.png" (There are several viral gene delivery systems, including adenovirus, retrovirus and lentivirus. Which one should I use for my experiments? 1) Retrovirus: requires active cell division. In addition, there is a significant risk of integration into the h...)
  • 20:43, 1 March 2018 Wendywilson (Talk | contribs) uploaded "File:Synthesize gene.png" (What is the difference between a long oligo and a synthetic gene? Answer: Due to limitations in oligo synthesis chemistry and other practical considerations, oligos are produced as mixture of single-stranded DNA strands and are limited in length to ~20...)
  • 20:42, 1 March 2018 Wendywilson (Talk | contribs) uploaded "File:Gene service.png" (How do you deliver synthetic genes? Answer: Creative Biogene will deliver 5.0ug plasmid DNA containing gene of interest in the vector and its bacterial stock. https://www.creative-biogene.com/Support/Gene-Synthesis.html)
  • 20:41, 1 March 2018 Wendywilson (Talk | contribs) uploaded "File:3 UTR Cloning.png" (Creative Biogene has developed an advanced vector-based miRNA expression plasmids, which can help to ease the difficulty in your research. These purified and ready to transfect miRNA expression plasmids can be used for a variety of applications to down...)
  • 23:51, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:Nucleoside Nucleotide.png" (Nucleic acids are organic polymeric macromolecules composed of nucleotide monomers building blocks. A nucleotide is made of nucleobase, a five-carbon sugar and 1-3 phosphate groups. Though the nucleotide normally refers nucleoside monophosphate, now nu...)
  • 23:50, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:Amino acid analysis service.png" (Hydrolysis is typically achieved under acid conditions. A standard procedure is hydrolysis with 6 M hydrochloric acid (24 hours, 110°C). Fragile amino acids, especially tryptophan and cysteine, will be partially destroyed. And then hydrolyzed samples ...)
  • 23:48, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:Sphingolipid Metabolism.png" (Cardiolipin (CL) is made up of two phosphatidyl moieties linked by a glycerol bridge. It is a tetra-acylated glycerophospholipid playing a crucial role in in both prokaryotic and eukaryotic cells. It is located at membranes associated with the producti...)
  • 23:48, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:Glycerophospholipids Analysis.png" (Glycerophospholipids are glycerol-based phospholipids. They are the main component of biological membranes. Glycerophospholipids present a significant challenge in high level of accuracy in lipid quantitation, given the headgroup diversity, large numbe...)
  • 23:45, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:Gene Editing Kits.png" (CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing. Besides the basic employment of gene knock-out, CRISPR/Cas9 system can be applied to selective gene ac...)
  • 23:32, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:Tn5 transposase sequence.png" (Robust Tn5 Transposase is a hyperactive form of Tn5 transposase. This enzyme can be used to randomly insert Tn5 transposon into target DNA. Robust Tn5 Transposase recognizes inside end sequences, outside end sequences and mosaic end sequences of Tn5 tr...)
  • 23:30, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:Cell lines knock out.png" (CRISPR/Cas9 system consists of a “guide” RNA (gRNA) and a bacterial CRISPR-associated endonuclease (Cas9). The gRNA is a short synthetic RNA composed of a Cas9-binding “scaffold” sequence and ∼20 nucleotide “targeting” sequence that defin...)
  • 23:29, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:Nuclear receptor screening.png" (Nuclear receptors (NRs) are a class of proteins responsible for sensing steroid and thyroid hormones and certain other molecules. There are 48 genes in the human genome that code for the NRs. The myriad molecular pathways modulated by NRs including but...)
  • 23:26, 30 January 2018 Henrrymo (Talk | contribs) uploaded "File:BAC Library.png" (Bacterial Artificial Chromosome (BAC) acts as a vector to artificially carry DNA into the cell of a bacterium, such as Escherichia coli, to make a BAC clone. In general, BAC clones can carry inserts of DNA up to 200,000 base pairs long. Recently, BAC i...)
  • 22:47, 1 January 2018 Henrrymo (Talk | contribs) uploaded "File:Acyl CoAs.png" ([https://www.creative-proteomics.com/services/acyl-coas-analysis-service.htm Acyl-CoAs] only exist inside the cells and their profiling inside the cells can give you some clues of the local metabolic status. Since each organ exerts its specific functio...)
  • 22:46, 1 January 2018 Henrrymo (Talk | contribs) uploaded "File:Sphingomyelin Profiling.png" ([https://www.creative-proteomics.com/services/sphingomyelin-profiling-service.htm Sphingomyelin profiling] service provides data on all individual molecular species. We recommend these profiling scans at the semi-quantitative level, which allows direct...)
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