Assaying Compound Activity
Depending on the project, assaying compound activity typically involves acquiring the compound and then getting it tested either in house or sending it out somewhere to be tested. This page details some of the common tasks and considerations in this process. Note, this information is somewhat specific to the Shoichet lab at UCSF.
Identifying Compounds to Buy
Depending on the project, compounds may have different requirements
- Requirements for in vitro testing
- Purity: If possible get the compounds at 95% or greater purity even if it costs 100$ or 200$ more. If it's tested to be active but has low purity, it will likely need to be re-purified and then tested.
- Aggregation: Check the compound with the aggregator advisor (advisor.bkslab.org) and if it's close to or known to be an aggregator (indications include uM nonspecific activity, logP great than 3 or actually shown to be an aggregator through appropriate controls) then either skip the compound or plan on doing aggregation controls, e.g. testing for detergent sensitive nonspecific activity, determine particle formation using DLS, or perform the assay with/without detergent (and just detergent controls).
- Amount: try to get 10mg if possible, but if it's very expensive you can get less. If you're sending it to a CRO sending it in solution usually requires less compound. If you're planning on further controls (e.g. for purity, aggregation), make sure to get enough.
- Note the solubility (e.g. water, DMSO, etc.)
- Requirements for in vivo testing
- Check pharmacokinetics
The easiest way to find vendors is through the ZINC portal. Once you login, go to Manage Carts in the upper right and then create new cart, where you put in a list of smiles or zinc ids. Once you've verified these are the right compounds, click purchasability report where it lists which compounds are available from which vendors. In selecting vendors keep the following in mind
- Here are some reliable vendors
- AK Sientific (in SF)
- Toronto Research Chemicals
- Sigma Aldrich (sometimes higher prices but reliable)
- Enamine (has a large catalog, in europe)
- ChemBridge (in SF)
- Santa Cruz Biotech (in CA)
- Some vendors are just resellers, which makes them have larger catalogs, but can increase the price and take longer. Though often this is offset by the ease of use and not having to pay multiple shipping and handling costs.
- MolPort (firstname.lastname@example.org has been the contact as of July 2014)
- If possible try to group multiple compounds per vendor to reduce shipping and handling costs
- Sometimes compounds are available from special catalogs
- NCI Platted: 1) Here is a description of the NCI Platted process from there end NCI_Platted. Look up the NCS identifier in PubChem. 2) submit a request form through their webpage. Contact the industry contacts officer at ucsf (Esteban.Mendoza@ucsf.edu | t: 415-502-1599) to have him sign the "Simple Letter of Agreement". He'll will give you a materials transfer form that you need to fill out.
- Remember that our lab will continue to buy from these vendors so be nice, and inquire if we can get a lab discount
- For each compound record
- ZincID, supplier, supplier product code, molecular weight, purity
- Get quotes either through the webpage or through vendor contacts
- Get Brian to approve the orders costing more than 500USD.
- Forward quotes to Yvonne or whoever is doing the ordering
- Be cognizant of the hours the orderer works, sometimes they share time with other labs
- the quote should including the zincid, vendor, vendor id, amount, and price
- lookup the project grant number (or ask Brian),
- a shipping address
- whose project this is for
- who should receive the compounds
- indicate if the order should be rushed (unless necessary, rushed orders should be avoided as they are generally much more expensive)
Testing Compounds with CRO
Here is a list of CROs .
- Quote administration
- If the vendor is not in the BearBuy system, they will need to fill out a form that Yvonne can provide
- Get a quote from the company and
- With the quote, get a PO number
- Give the PO number to the vendor when placing the order
- The vendor will then give an Invoice that can go directly to the UCSF Accounts Payable department (COapmail@ucsf.edu) and they will pay the vendor.
- Sending compounds
- Provide the courier (e.g. FedEx) with the address and full name and telephone number of the person who will receive it.
- Indicate with the vendor if the order should be rushed (unless necessary, rushed orders should be avoided as they are generally much more expensive)
- If the supply of the compound is limited, often less is required if it is sent in solution, but it is easier and more stable to send powder, so check with the assay vendor
- Compound testing
- For the given receptor/ligand pair check the known activities to help guide where to start testing.
- If possible, start with a few high concentration (e.g. 2 replicates @ 20 uM) "primary" points to see if there is any activity
- If there is some activity follow up with a 5-10 point dose response curve reducing by half log or 1/3 at each step (where the highest concentrations will depend on the activity of the primary assay).